Guts of the collected organism were separately crushed in 0. The steps of this study consisted of cellulose isolation, cellulolytic bacteria isolation and identification. Despite this vast number of cellulase producers, there is a deficiency of microorganisms that can produce significant amount of the three cellulase enzyme specifities i. The macerated gut of the collected organisms was inoculated in a basal salt media nano 3 2. Isolation and identification of cellulose degrading. New procedures have been developed for the isolation and purification of aerobic and facultatively anaerobic bacteria able to utilize cellulose as sole source of carbon and energy. This has been confirmed from the results of isolation of cellulose degrading bacteria that produce clear zone beneath and around the colonies. Isolation and screening of cellulose degrading bacteria. Isolation and identification of cellulose degrading bacteria from different sources at assiut governorate upper egypt. A total of 245 cellulose degrading aerobic bacterial strains were isolated from different natural reserves in the subtropical region of china, which were cultured in agar medium containing sugarcane bagasse pulp as the sole carbon source.
The aim of this study is to isolate and characterize bacteria able to degrade starch from the rhizospheres of various plants at four. Cellulolytic microorganisms such as fungi and bacteria are responsible for much of the cellulose degradation in soils. Screening, purification and characterization of cellulase. Cellulases are now used in the textile industry for cotton softening and denim finishing, in laundry. Cellulase which produced by bacterial isolates are extracellular enzyme 1. This method was first described by hungate in 1947 and is still extensively used today, and widely regarded as the best method for the isolation of obligate anaerobic cellulose degrading bacteria and fungi that cannot be cultivated on solid medium in petri dishes. Results and discussion cellulose degrading bacteria were isolated from mangrove soils of mahanadi delta using cmc agar medium figure 1.
Eight isolates of cellulosedegrading bacteria cdb were isolated from four different invertebrates termite, snail, caterpillar, and bookworm by enriching the basal culture medium with filter paper as substrate for cellulose degradation. Isolation of cellulosedegrading bacteria and determination of. Pdf isolation and identification of cellulose degrading. Request pdf isolation of cellulosedegrading bacteria the details of many microbial processes can only be clarified by using pure cultures. Amylase activity of a starch degrading bacteria isolated. These tissues are monitor organs that usually harbor the diseasecausing bacteria during infection. Pdf isolation and characterization of potential cellulose. Nuagada, atharabanki and mangrove forest at indian farmer. Isolation and screening of cellulose degrading microorganisms. Isolation and identification of cellulose degrading bacteria. India is the fifth largest producer of pineapple with an annual output of about 1. In this way cellulose degrading bacteria were isolated from these six positive samples and bacteria were identified as two species of thermoactinomycetes sp. Optimization and molecular identification of novel. Isolation and characterization of cellulolytic bacteria from.
About one fifth of fresh water and soil samples yield cellulose degrading bacteria after enrichment but some samples did not bear such kind of bacteria 17. Isolation and characterization of starch degrading rhizobacteria. Isolation, screening and molecular identification of. This method was first described by hungate in 1947 and is still extensively used today, and widely regarded as the best method for the isolation of obligate anaerobic cellulosedegrading bacteria and fungi that cannot be cultivated on solid medium in petri dishes. Methods for the isolation of cellulosedegrading microorganisms. Isolation of cellulose degrading bacteria from rumen and evaluation of cellulase production by the isolate using lignocellulosic substrate submission of manuscript for publication olyad erba urgessa haramaya university, college of natural and computational sciences, school of biological sciences and biotechnology. Studies on cellulose degrading microorganisms associated with rumen of ruminants was carried out from ruminants ram, cow, and goat, through culture, microscopic identification, biochemical test and cellulose degrading methods.
Isolation of cellulosedegrading bacteria and determination. Bushnell haas medium bhm was used for isolation of cellulose hydrolytic bacteria after amendment with carboxymethylcellulose cmc as the sole carbon source. Ten grams of each soil or ward poultry sample were diluted with 90 ml saline solution in erlenmeyer flasks. Fortyone cellulose degrading bacteria cdb were isolated from soil samples from seven different paper and pulp mill dump sites using enrichment method.
Isolation of cellulosedegrading bacteria request pdf. This has been confirmed from the results of isolation of cellulosedegrading bacteria that produce clear zone beneath and around the colonies. Isolation and characterization of potential cellulose. Functiondriven singlecell genomics uncovers cellulose. Isolation, screening and characterization of cellulolytic. Cellulose feeding organisms like termite, caterpillar, bookworm, and snail were collected for isolation of cellulosedegrading bacteria from woody habitats. Isolationofcellulosedegradingbacteriaanddeterminationof. Isolation and screening of cellulosedegrading bacteria. Bacteria are usually isolated from fish kidney and spleen.
Isolation and identification of cellulose degrading bacteria from. International journal of applied microbiology science, 12. A total of 245 cellulosedegrading aerobic bacterial strains were isolated from different natural reserves in the subtropical region of china, which were cultured in agar medium containing sugarcane bagasse pulp as. Isolation and characterization of cellulosedegrading. Aug, 2018 since cellulose is the most important component of plant biomass and a source of carbon, the cellulose degrading microorganisms cdms were isolated from these litter falls and screened for their ability to degrade carboxymethyl cellulose cmc in agar plates as substrate. Recent advances in biodegradation, sanitation, and bioremediation. Cellulose degrading bacteria can be used for commercial production of cellulase enzyme in which the enzyme is extracted by the use of bioprocessing technology from cellulolytic bacteria 1,2,3. Cellulose degrading bacteria from termite cryptotermes sp. Isolation, characterization and practical significance of cellulose degrading bacteria from the gut wall of two ecologically distinct earthworms kishore k. The isolates were identified based on morphology and biochemical characterization while the identities of the potent. Among 12 isolates of bacteria, six isolates were cellulolytic. In the present study, cellulose degrading bacteria was isolated from sheep rumen. Isolation and characterization of potential cellulose degrading bacteria from sheep rumen article pdf available in journal of pure and applied microbiology 3.
The biochemical characterizations of the isolated strains c 1, c 2 and c 3 are presented in additional file 3. Jan 01, 2017 how to isolate cellulose degrading bacteria sajid shaikh. Potential isolates with cellulase production were identified by. Fallow period of a jhum cycle is the recuperation phase of jhumming practice, where most of the processes like regeneration of secondary forest, equilibration of biomass forest litter fall decomposition and nutrient cycling processes take place. Isolation of cellulose degrading bacteria from rumen and. After 21 bacterial strains were isolated, the one with cellulose degrading ability were determined using enrichment technique. Cellulolytic organisms can also be used as probiotics in animal nutrition. Biomass decomposition is considered to be the key process that supports all other biogeochemical processes. All bacterial culture showed growth as the medium turned cloudy and the. Isolation and preliminary screening of lignin degrading microbes. Cellulose degrading bacteria were enriched and isolated by inoculating filter paper in liquid medium with macerated guts from termite, bookworm, snail, and caterpillar separately.
Isolation and characterization of starch degrading. Mesophilic aerobic gram negative cellulose degrading. Enrichment technique was used for isolating cdb cellulose degrading bacteria. Isolation and preliminary screening of lignin degrading. Therefore, the present investigation was designed to isolate and screen the cellulase producing bacteria from soil. Studies on cellulose degrading microorganisms associated with.
Pdf eight isolates of cellulosedegrading bacteria cdb were isolated from four different invertebrates termite, snail, caterpillar, and bookworm. Thatoi, isolation and identification of cellulose degrading bacteria from angrove msoil of mahanadi river delta and heir tcellulase production ability. Isolation, biochemical characterization, and phylogeny of. Isolation and screening of cellulolytic bacteria from soil. A microscopic examination revealed that the isolated strain c 1 and c 2 was rod shaped and found to be gram positive where as c 3 strain was short rod in shape and found to be. Studies on cellulose degrading microorganisms associated. Cellulolytic activity of cellulose degrading bacteria on cmc medium was assayed using degradation of whatmann no. Isolation, screening and identification of cellulase producing bacteria. Isolation and characterization of cellulose degrading bacteria of termite gut from north eastern region of india special issue on. Pdf isolation of cellulosedegrading bacteria and determination. Isolation of bacterial strains streaking plate method was used for obtaining pure bacterial strain.
This report is submitted in partial fulfillment of the requirements for the degree of bachelor of science with honours resource biotechnology faculty of resource science and technology university malaysia sara w ak 2006. Isolation of cellulose degrading bacteria the soil samples were collected from different location of mangrove forest such as jumbo, kharnasi, triveni, 42 american journal of microbiological research. Isolation and screening of cellulases producing bacteria. Peters college, kolenchery, kerala, india received june 02, 2016, accepted june 29, 2016 abstract. This is due to existence of microenvironments where different growth conditions for cellulose degrading bacteria are present. Isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion.
Taken together, these data provided support for the efficacy of our approach and its utility for the targeted isolation of environmental bacteria that bind cellulose. Fortyone cellulosedegrading bacteria cdb were isolated from soil samples from seven different paper and pulp mill dump sites using enrichment method. Isolation, screening and characterization of cellulolytic bacteria from forest soil sample korra ashwani1, lavudi saida1 and k. How to isolate cellulose degrading bacteria sajid shaikh. Pdf isolation and characterization of cellulolytic bacteria. Isolation, screening, and identification of cellulolytic.
Isolation, biochemical characterization, and phylogeny of a. Cellulose degrading bacteria were isolated from mangrove soils of mahanadi delta using cmc agar medium figure 1. Isolation of cellulolytic bacteria, including actinomycetes, from coffee exocarps in coffeeproducing areas in vietnam, international journal of recycling of organic waste in agriculture, 2014, pp. Starch degrading bacteria are most important for industries such as food, fermentation, textile and paper. Isolation is done to obtain pure bacterial cultures. Comparison of detachment procedures for direct counts of bacteria associated with sediment particles, plant litter and epiphytic biofilms. Isolation and screening of cellulose degrading microbes. The number of cultivable bacteria per gut was calculated from the average number of colonies present on primary isolation plates pelczar et. Isolation and screening of cellulose degrading bacteria in. Materials and methods isolation of bacteria bacteria were isolated from the soil sample collected from botanical garden.
Isolation and identification of cellulose degrading bacteria and optimization of the cellulase production joseph vimal, akhil venu, and jini joseph department of biotechnology, st. Isolation of cellulosedegrading bacteria springerlink. Anand aap, vennison sj, sankar sg, probhu dig, vasan pt, raghuraman t, et al. Gupta p, samant k, sahu a 2012 isolation of cellulosedegrading bacteria and determination of kadarmoidheen m, saranraj p, stella d 2012 effect of cellulolytic fungi on the degradation of cellulosic agricultural wastes. A type of cellulase, endoglycosidase cleaves the disaccharide cellobiose from cellulose, and another type of enzyme. Lignocellulose is the major structural component of woody plants and nonwoody plants such as grasses and represents a major source of renewable organic matter. Essentially, the choice is between insoluble forms of cellulose, the degradation of which is a true indication of cellulolytic activity, or soluble derivatives such as carboxymethyl cellulose cmc, which is convenient to incorporate in isolation media, but can be degraded by many microorganisms that produce endoglucanases in the absence of significant activity against native cellulose. Isolation and screening of cellulose degrading bacteria in kitchen.
Isolation and identification of cellulolytic bacteria from. Isolation and identification of cellulolytic bacteria from the gut of. Venkateswar reddy2 1centre for biotechnology, institute of science and technology, jawaharlal nehru technological university, hyderabad 500085, t. Abstract this study was conducted to isolate cellulose degrading bacteria from mangrove soil of mahanadi river delta, odisha, india and also to evaluate their. The identification is required so as to cure the illness or the infection caused due to. The isolates were identified based on morphology and biochemical characterization while the identities of the potent cellulolytic bacteria isolates were confirmed by 16srrna sequencing. Screening isolation and characterization of cellulase. Isolation of cellulose degrading bacteria from rumen and evaluation of cellulase production by the isolate using lignocellulosic substrate.
All bacterial culture showed growth as the medium turned cloudy and the filter paper became macerated. The cellulase producing bacteria were isolated from industrial and agricultural areas in kerala. Cellulose degrading bacteria were enriched and isolated by inoculating. Optimization and molecular identification of novel cellulose. Starch degrading bacteria are important for different industries such as food, fermentation, textile, and paper. Isolation and characterization of cellulase producing. Enrichment and isolation of mesophilic 37c and thermophilic 60c cellulosedegrading bacteria weathered soillike materials were used to isolate cellulose degrading mesophilic and thermophilic bacteria.
421 697 1292 854 1039 1231 661 1472 434 1365 1572 1597 1163 1079 1502 411 810 1118 832 917 645 717 880 1198 527 1303 1136 1091 848 608 1551 213 1198 1349 511 1275 1466 1222